| 1. What are porins? What role do they play in diffusion? |
Porins are protein that form internal channels in outer membrane of Gram negative bacteria and some eukaryptic cells. They facilitate diffuciosn by allowing specific molecules to pass through, regulating solute transport and maintaining cellulear homeostasis. |
| 2. Where does the Pholoysis of H2O occur? What is its significance? |
Pholoysis of H2O occurs in the thylakoid lumen of chloroplasts. Its significance is that it leads to the formation of ATP and NADPH, which are essential energy-rich molecules for the subsequent dark reactions of photosynthesis. |
| 3. What are pleomorphic bacteria? Give examples. |
Pleomorphic bacteria are those that lack fixed shape and can occur in various forms. Eg: Mycoplasm |
| 4. What is point mutation? Give an example. |
Point mutation is a type of mutation where a single nucleotide base is substituted with another in a DNA sequence. Eg: a change from ATCG from ATGG, where cytosine is replaced with gguanine. |
| 5. What is meant by charging of a tRNA? |
Charging of a tRNA refers to the attachment of a specific amino acid to its corresponding transfer RNA molecule, catalyzed by aminoacyl-tRNA synthetase enzyme. |
| 6. Distinguish between heterochromatin and euchromatin. Which of the two is transcriptionally active? |
Heterochromatin is densely packed, transcriptionally inactive chromatin, whereas, euchromatin is loosely packed transcriptionally active chromatin. Euchromatin is transcriptionally active form allowing gene expression to occur. |
| 7. Give one example for each of transgenic plants which are suitable for food processing and those with improved nutritional quality. |
Transgenic plant for suitable food processing - Golde rice with modified starch for easier processing.
Transgenic plant with improved nutritional quality - Golden rice with enhance beta-carotene (Provitamin A) content to combat vitamin A deficiency.
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| 8. What is the full form of PCR? How is it useful in biotechnology? |
PCR stands for Polymerase Chain Reaction. Its useful in biotechnology for amplifying specific DNA sequences, allowing for the rapid generation of multiple copies of a gene or DNA fragment which can be used for cloning, sequencing, and genetic engineering. |
| 9. Why does swiss cheese has big holes? Name the bacteria responsible for it. |
Swiss cheese has big holes due to the production of carbon dioxide bacteria during fermentation. The bacteria responsible for this is Propionibacterium Freudenreichii. |
| 10. Give two example of fungi used in SCP production. |
1. Aspergillus
2. Fusarium
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